Diagnostic model of visceral leishmaniasis based on bone marrow findings. Study of patients with clinical suspicion in which the parasite is not observed

Published: 26 August 2019
Author(s): Alfredo Bermejo Rodriguez, Jose Manuel Ruiz Giardin, Jesus Garcia Martinez, Juan Victor San Martin Lopez, Adela Castaneda de la Mata, Daniel Lopez Lacomba, Jeronimo Jaqueti Aroca, Stefan Walter
Issue: August 2019
Section: Original article

Visceral Leishmaniasis (VL) is a serious protozoal disease endemic in diverse areas, including the southern area of Madrid (Spain), where an outbreak was detected in 2009. The objective of this work is to analyze bone marrow alterations in VL patients and elaborate a diagnostic model with the aim to improve the early detection of this disease. The usual diagnostic methods, as the observation of the parasite on a bone marrow aspirate, have frequent false negatives, and the high sensitivity methods, as PCR and ELISA, are delayed or are not always available.