Little is known on Lyme borreliosis (LB) in patients aged ≥65 years. To optimize management of older patients referred to a tertiary Lyme centre, characteristics of older patients need to be assessed. We published a study on depressive symptoms in patients referred to a tertiary Lyme centre [1]. Following that study, we now assessed prevalence of LB and prevalence of depressive symptoms in patients suspected of LB aged ≥65 years compared to 18–64 years old.