The aim of the study was to evaluate the health effects associated with the exposure to ground-level of particulate matters with aerodynamic diameter ≤ 2.5 μm (PM2.5) on citizens in Rome (Italy) in 2015 and 2016. Based on the new version of the World Health Organization's AirQ+ model, we have estimated the short- and long-term effects of PM2.5 on hospital admissions due to cardiovascular (HA-CVD) and respiratory diseases (HA-RD) as well as on mortality for ischemic heart disease (M-IHD) and chronic obstructive pulmonary disease (M-COPD).